Injection technique for indocyanine green and sodium fluorescein dye angiography of the eye.

This study was done in an effort to improve the intravenous dye injection technique used in fluorescent indocyanine green and sodium fluorescein dye angiography of the eye. The concentrations of these two dyes in blood at which maximum fluorescence occurs were determined, and methods of achieving these concentrations in the ocular vasculatures via cubital vein injection were investigated. The effects on arterial dye concentration produced by changes in the concentration and volume of intravenously injected dye boluses and use of saline flushes were investigated using monkeys and human subjects. Based on the experimental data, an intravenous injection technique is suggested for improving the quality of fluorescent ocular angiograms.